Journal: GigaScience

Article Title: StereoSiTE: a framework to spatially and quantitatively profile the cellular neighborhood organized iTME

doi: 10.1093/gigascience/giae078

Figure Lengend Snippet: Spatial transcriptomic mapping of xenograft model in situ . (A) Flowchart illustrating the construction of the xenograft model is presented. BALB/c mice were subcutaneously injected with CT26 cells derived from colon cancer. Subsequently, the mice were either treated with a vehicle or DMXAA for a period of 14 days posttumor transplantation. Tissue samples were collected 24 hours after the treatment. (B) Heatmap depicting the expression of transcriptional markers of 11 annotated immune cell types at a single-cell resolution is shown. (C) An analysis of the proportion of immune cells at a single-cell resolution in each sample is provided. (D) Histogram comparing nonimmune cells to immune cells is displayed, where the blue bar represents the proportion of nonimmune cells, and the gray bar represents the integrated proportion of immune cells. The numbers associated with each bar indicate the precise cell counts for each sample. (E) Representative hematoxylin and eosin staining images of sample 717 from the control group (left) and sample 718 from the treatment group (right) are presented. The red-thread restricted region highlights the necrotic site. (F) In situ visualization of annotated cell types using stereo-seq data is shown on the left, an enlarged image of the marked-circle-site displaying cellular compositions is in the middle, and representative immunohistochemistry staining (CD163 indicating macrophages in sample 717 and Ly6G indicating neutrophils in sample 718) of the same marked-circle-site is presented on the right.

Article Snippet: The prepared section underwent processing in accordance with the Stereo-seq Transcriptomics Set User Manual (STOmics), utilizing reagents from the Stereo-seq Transcriptomics T kit and Stereo-seq Library Preparation kit (STOmics).

Techniques: In Situ, Injection, Derivative Assay, Transplantation Assay, Expressing, Staining, Control, Immunohistochemistry

Journal: bioRxiv

Article Title: Spatiotemporal transcriptomic niches of complement pathway and serine protease inhibitor activation in aging and infection

doi: 10.1101/2024.11.04.621811

Figure Lengend Snippet: a , Visualization of the five Bregmata selected to study different regions of the aging brain. b , From left to right: Two-dimensional UMAP representation of colored spot clusters computationally integrated by brain slice (top to bottom), pie chart showing the proportion of annotated clusters across all brain samples, one representative annotated Visium sample with the spot cluster identities plotted over the H&E-stained tissue image. c , Number of differentially expressed genes per aging brain bregma (old vs. young) and direction of dysregulation. Total DEG counts were derived across all organ clusters, without removing duplicates. d , Five-dimensional Venn diagram comparing the brain DEG sets from ( c ). e , Heatmap showing scaled expression of the 17 aging DEGs (rows) shared between all five brain slices, using the Brain1 pseudobulk samples and spot clusters for visualization (columns). All genes except Rbm3 are also significant SVGs in at least one of the five brain bregmata. f , Sketch of 10x Visium and STOmics Stereo-seq examples comparing the features of both spatial transcriptomics technology platforms. g , Examples for binned (bin200) and annotated spot clusters of the aging brain (top to bottom; young, middle, old) at Bregma#1 sequenced with Stereo-seq. h , Dot plot showing the top 3 most significant marker genes per cell type annotated spot cluster using the Stereo-seq cellbin resolution of Brain1 samples. i , Normalized spatial expression of Trem2 across all 15 STOmics Stereo-seq brain samples using the near-cellular resolution bin20 (from left to right: young, middle, old; from top to bottom: Brain1-5). For visualization spot sizes were rescaled into the point interval [0.1, 1.5] according to their expression of Trem2.

Article Snippet: One brain sample of each age was processed at the MGI Tech Co., Ltd. (Riga, Latvia) using the STOmics Stereo-seq Transcriptomics T Kit (MGI).

Techniques: Slice Preparation, Staining, Derivative Assay, Expressing, Marker

Journal: bioRxiv

Article Title: Spatiotemporal transcriptomic niches of complement pathway and serine protease inhibitor activation in aging and infection

doi: 10.1101/2024.11.04.621811

Figure Lengend Snippet: a , Illustration of the five different brain bregma used for STOmics Stereo-seq in accordance with the Visium data set. Representative H&E stains are shown for each Bregma. Since Stereo-seq does not support H&E stains directly from the sequenced tissue slices, an adjacent (directly before or after) tissue slice was prepared and stained before running the spatial transcriptomics experiments. b , From left to right and per brain bregma (top to bottom): integrated UMAP representation of all cleaned Stereo-seq spot clusters using the bin200 resolution, pie charts and per replicate spatial projections of the final annotated spot clusters. Cluster names and colors were assigned in accordance with the Visium data set (cf. Methods). c , Distribution of four main quality control features across the cleaned spots and per Stereo-seq brain replicate at bin200 resolution.

Article Snippet: One brain sample of each age was processed at the MGI Tech Co., Ltd. (Riga, Latvia) using the STOmics Stereo-seq Transcriptomics T Kit (MGI).

Techniques: Staining, Control